Sentence examples for procedure and sequenced from inspiring English sources

All other PCR products were cleaned (same procedure) and sequenced directly (both directions) with their respective PCR primers (Table 4).

Genomic DNA from Enterococcus faecalis isolates were prepared from overnight cultures using a standard phenol-chloroform extraction procedure and sequenced by Illumina MiSeq 2000.

For ChIP-seq, libraries were generated from ChIP genomic DNA samples according to the Illumina ChIP-seq library construction procedure, and sequenced using Illumina Genome Analyzer IIx with a read length of 36 or 76 bp.

The exons and the 5′ region of intron 1 of KIT were amplified using a touchdown procedure and sequenced as described previously (Table S5) (Ishida et al. 2006).

PCR products from nematode homogenates were cloned into pGEM-T Easy (Promega), transformed into E. coli XL1-Blue, extracted, amplified and purified according to standard procedures, and sequenced as described below.

We amplified the p17 gag gene (396 nucleotides) and a partial fragment of the gp41 env gene (360 nucleotides) of the HIV genome with nested polymerase chain reaction (PCR) procedures and sequenced them.

Primer design and sequence analysis were performed in CLC Main Workbench (CLC bio, Aarhus, Denmark); the sequencing was carried out using standard procedures and sequenced on the ABI3130XL (Life Technologies Ltd., Paisley, UK) as previously described [ 2].

Single-end cDNA libraries were prepared for each sample and sequenced using the Illumina TruSeq RNA Sample Preparation Kit by following the manufacture's recommended procedures and sequenced using the Illumina HiSeq 2000.

We amplified cytochrome b mitochondrial DNA (≈1,140 bp) with primers L14724 and Citb-rev by using standard PCR procedures and sequenced the samples with the same primers and an additional internal primer MVZ16 (6 ).

Normalized cDNA libraries from the polyadenylated RNA fraction from nine of the mixed stage nematodes were constructed at the GSC at Washington University by standard procedures and sequenced on the Roche 454 FLX platform producing 320,000 to 540,000 reads.

However, this estimate is continuously increasing as advances in virus isolation procedures and sequencing methods impact on virus studies.