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The nebulizer procedure and image acquisition were performed in the same room in the Nuclear Medicine Department.
To do that, we used the same procedure and image analysis system as described above for NeuN immunopositive cells.
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For NO fluorescence, epidermal peels were loaded with 10 μM of the NO-sensitive dye diaminofluorescein diacetate (DAF2-DA, Calbiochem, UK) using exactly the same dye loading procedure, and images acquired using confocal microscopy as described above.
This model proved to be problematic as it led to issues of food quality, procedures and image management.
Immunocytochemical and immunohistochemical procedures and image acquisition were performed as previously described [18], [33] (Table S1).
Western blots were conducted by standard procedures and imaged on a BioRad ChemiDoc imager using chemiluminescence.
The use of spikes allows not only data normalization but also the evaluation of several parameters of the platform quality, including the sensitivity and specificity of the microarray experiments, the accuracy and reproducibility of the measurements and the assessment of technical variability introduced by labeling procedure, hybridization and image scanning [ 7, 8].
Ultrathin sections were obtained following standard procedures and imaged in a Hitachi H-7650B microscope (Tokyo, Japan).
We retrospectively reviewed all cases and excluded non-tumour specimens, lesions that had undergone previous surgical procedures and images that were misregistered or of poor quality (unfocused or motion blurred).
Figure 3 Synthetic procedure and TEM images and size distribution of Pd NPs.
The validity of the calibration procedure and the images' reproducibility were tested by capturing sample images in three different lighting conditions: dark, medium and intense lighting.
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