Sentence examples for procedure and digested from inspiring English sources

Genomic DNA from individual regenerants was isolated using a CTAB extraction procedure and digested with HindIII.

In the BAC end cloning procedure, 30 ng of BAC DNA were isolated through standard miniprep procedure and digested with Not I and Dra I and then ligated to Not I and EcoRV sites of pPCR-script AMP SK vector (Stratagene).

After this pre-treatment, the DNA was put through the mapping array procedure and further digested with either the Nsp I or Sty I restriction enzyme.

Briefly, the left and the right homologous arms were amplified by standard PCR procedure before digested and inserted into the pBSK vector.

The oligos were annealed using standard procedure and ligated to pDest GFP-Tus digested with NheI and MunI.

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A procedure has been developed for the simultaneous determination of traces amounts of Cd, Cr, Cu, Mn, Ni and Pb from saline oil-refinery effluents and digested vegetable samples using inductively coupled plasma optical emission spectrometry (ICP OES).

The agarose was melted at 68°C and digested with ß-agarase (New England Biolabs, Hertfordshire, England).

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For phage-display library construction, 5 µg of SfiI-NotI-digested plasmid pHEN2 and digested fragment were ligated in a 1∶5 ratio during 16 hs at 16°C.

Agarose embedded DNA was prepared and digested with PmeI and BamHI as previously described [18].