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In a second procedure, a threshold was applied to each image.
In order to separate the fluorescence intensities between the object (bead) and the background during the image segmentation procedure, a threshold value needs be established to cutoff the background pixel values.
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As part of the detection procedure, a threshold-based gradient method is used to highlight long linear segments or to enhance short linear ones.
For the screening procedures a threshold score of ≤ 5 yielded the maximum clinical efficiency with a sensitivity and specificity of 90.9% and 17.6% respectively.
This procedure includes a threshold filter to eliminate random procedural errors followed by partial sequence assembly to cross-validate observed patterns of variation in words.
The distribution of AUC values obtained after the iterative procedure suggests a threshold for hybrid detection equal to 0.84 (data not shown).
Selection was carried out using a stepwise procedure with a threshold of entry of 20%.
The mFAVN test is a quantitative procedure requiring a threshold to separate positive from negative results.
To select significant interactions, a backward elimination procedure with a threshold of P < 0.20 was used.
A significant level <0.10 was used to select the variables for the Cox's proportional hazards model, using a stepwise backward procedure with a threshold of a=0.05.
Regarding hospital procedure volumes a threshold of 100 operations annually has been chosen because this threshold has already been used by Katz et al. in their prominent study on the association between procedure volumes and outcomes [ 13].
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