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In supercritical fluid extraction supramolecular solvent microextraction procedure, a mixture of decanoic acid and the SFE collecting solvent (tetrahydrofuran) was added to water for supramolecular solvent formation.
General procedure A mixture of compound 3 (0.368 g, 1 mmol) and the appropriate hydrazonoyl chlorides 4a g or 10a d or 16a, b (1 mmol) in ethanol (20 mL), triethylamine (0.1 g, 1 mmol) was added.
In a typical procedure, a mixture of 135 mL MnSO4 solution (1.75 mol/L) and 13.6 mL concentrated HNO3 (16 mol/L) was added quickly to 450 mL of boiled KMnO4 solution (0.04 mol/L).
In a typical experimental procedure, a mixture of barbituric acid 1a,b, dimedone 2 and aromatic aldehyde 3 in water was stirred in the presence of a stoichiometric amount of diethylamine (1.0 equiv).
General procedure A mixture of thione (3) (0.343 g, 1 mmol), 37% formaldehyde solution (2 mL) and the appropriate aniline derivative (4a g) (1 mmol) in dioxane (20 mL) in the presence of few drops of HCl was stirred at room temperature for 4 8 h (monitored by TLC).
A closed cylindrical Teflon-lined stainless autoclave was used in the following procedure: a mixture was made of 21.2 ml of titanium isopropoxide and 4 ml of acetic acid, which was agitated at 300 rpm for 15 min at room temperature.
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Herein, NiCo2O4 double-shelled hollow spheres were synthesized via a microwave-assisted, fast solvothermal synthetic procedure in a mixture of isopropanol and glycerol, followed by annealing.
The extraction of HMs from soil samples were made by the wet procedure with a mixture of mineral acids (HCl, HNO3, 3 1 ratio) and from vegetables by plant ash digestion with 0.5N HNO3.
Anaesthesia was induced and maintained throughout the procedure with a mixture of isoflurane and medical air.
When applying the extraction and purification procedure to a mixture of 13 POPs, we noticed that 76 ± 3% of the initial estrogenic activity was recovered.
The second follows the Folch procedure using a mixture of chloroform: MeOH (2 1) [ 42] and the third, from Hara et al. [ 43], uses a mixture based on n-hexane:isopropanol (3:2).
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