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In order to assess whether cells generated under the influence of oestradiol survive over a long period of time, pups were treated with oestradiol and BrdU on PN0 and PN1, then sacrificed on PN21 (for procedural timeline see Figure 1C).
In order to assess the short- and long-term survival of newborn cells in the neonatal hippocampus, pups were treated with oestradiol followed 2 h later with a single injection of BrdU on PN0 (for procedural timeline see Figure 1-B).
In order to assess the effects of oestradiol on cell proliferation in developing hippocampus, pups were treated on PN0 with oestradiol followed 2 h later with a single injection of BrdU and euthanized 6 h post-BrdU injection (for procedural timeline see Figure 1-A).
In order to assess the impact of endogenous oestradiol on cell proliferation, we quantified the number of BrdU+ cells in pups that were treated on PN0 and PN1 with formestane (an aromatase inhibitor), tamoxifen (an oestrogen receptor (ER) antagonist) or vehicle, followed 2 h later with an injection of BrdU (for procedural timeline see Figure 1D).
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Before, states simply reported how well they met procedural requirements such as timelines for evaluations or due process hearings.
A commitment to a procedural framework of resource allocation, timelines for decision-making and clear, respectful communication at every stage of the process are likely to dispel the perceptions outlined.
Timeline of experimental procedures.
In the third medical school year, students learned to identify general indications for specific focused ultrasound examinations and the proper timeline in the clinical scenario for their use as well as gaining a deeper understanding of ultrasound for procedural guidance.
Timeline: 8.40am.
This timeline.
The Urban-Decay Procedural?
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Justyna Jupowicz-Kozak
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