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However, gene therapy is sometimes not feasible for mucosal drug delivery, because of the intrinsic problems of the approach (that implies genetic manipulation of human cells and their re-introduction into the body) and of those related to the choice of the appropriate target cells.
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One of the main problems of this approach is its availability and it can bring the entire MANET to a halt if it moves out of the MANET.
In response to several requests that I elaborate further on the problems of this approach and a possibly more constructive approach, I offer the following.
The problems of this approach are multiple.
University officials say it creates a "more positive" learning environment.But the practical problems of this approach are obvious.
Therefore, researchers proposed another approach, called DPM [19], to overcome some of the problems of the PPM approach.
Main problem of the approaches [17,33,34] is that it is not able to generate revocable key for session based communication.
This is so due to steric problem for the approach of the OH- to the carbon bearing the chlorine atom.
Some principal problems of the classical approach for parametrical identification are discussed.
The example of the business traveler highlights one of the serious problems of the averaging approach.
We will then highlight some problems of the reductionist approach and discuss how these may be overcome using systems biology.
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