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Unfortunately, the problems of expression and purification of E. coli produced recombinant proteins are atypical.
These problems were due to; "Lack of common terminology", "Subjective anxiety on evaluation of spatial quality and quantity", "Difficulties in expressing positive, negative and missing parts of student studies, through the design process assessments", "Problems of expression during information exchanges between project groups, etc".
According to the comments from the experts, there was a need for generalization and specification of some items, as problems of expression were identified (see Additional file 1).
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Overcoming the solubility problem of expression eukaryotes protein in E. coli is always a challenge (Correa and Oppezzo 2015).
In 1968 he published Difference and Repetition as his primary thesis for the doctorat d'Etat, with Spinoza and the Problem of Expression as the secondary thesis.
While the lack of activity of PR-3 was due to a problem of expression (Fig. 4A) that might be consequent to the presence of the structurally destabilizing L64P mutation [38], the reduced activity of PR-2 may be related to the S79G substitution, previously shown as deleterious for this activity [33], [34].
Several approaches have been taken to the problem of expression deconvolution and each approach depends on access to different types of information, different statistical assumptions and different objectives.
To bypass problems of low expression levels with the pcDNA constructs, most of the SVSP constructs were subcloned into the pmaxCloning expression vector (Amaxa) using standard protocols, resulting in much higher SVSP expression levels in mammalian cells.
They avoided political or religious commitment and shared an often skeptical outlook and an interest in problems of lyrical expression.
The interaction, definition, and redefinition of media and problems of artistic expression are key to this project, which cuts across disciplinary fields in art history and visual culture.
The theater has also been having problems of artistic expression.
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