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Moreover, stiffness can lead to a variety of problems for methods such as bifurcation or sensitivity analysis.
The lack of haplotype information presents particular problems for methods designed to detect recombination through comparisons of sequences from different individuals, using the coalescent theory (e.g. McVean et al., 2002; Fearnhead et al., 2004) or phylogenetic break-point methods (Minin et al., 2005).
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The use of such multiplex measurements will itself generate significant problems for method validation, such as crossreactivity, interference, sensitivity and stability.
This poses particular problems for distance methods.
This could be a problem especially for methods using antibody-based salmonella detection strategies, if the antibodies are not sufficiently specific.
Pettazzoni, in his concern for problems of method, was critical of the sharp division between phenomenology and history.
NCI calculations have been a serious problem for DFT methods.
This is the same problem for all methods of standardisation.
This weakness is a problem for all methods based on recommendation techniques.
However, we conclude that divergences in mitochondrial sequences above super-order levels represent an insurmountable problem for current methods.
Missing answers on factors are a problem for the methods that allocate participants based on their specific values (e.g., stratification but not randomization).
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