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Alignment against the rice RefSeq identified one misassembled contig caused by a single problematic read.
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"In sum, the evaluators and the Committee found that temperament issues, particularly bias and lack of open-mindedness, were problematic," reads a statement by Pam Bresnahan, the chair of the ABA's standing committee that reviews nominees.
A procedure called "flowgram clustering" removes problematic reads and increases the accuracy of the taxonomic analysis.
Problematic reads can arise from several stages of long-insert library construction.
Occasions can arise where correct reads suggest joining of two scaffolds but problematic reads suggest otherwise, in which case the SSPACE scaffolder will scaffold correctly because the number of correct reads outweighs problematic reads.
Similarly, Newbler 2.3 and Newbler 2.5 generate separate lists of singletons, repetitive reads, and 'outliers' (problematic reads such as chimaeras).
An explanation is that our long-insert libraries were sequenced at adequate coverage and problematic reads were present in minority.
Reads sequenced from longer fragments are still useful in scaffolding and problematic reads can be detected and filtered out as long as they remain a minority.
For mcrA diversity evaluation, however, we only analysed high-quality sequences (no chimaeras or frameshifts), and all the problematic reads were subsequently excluded.
Not only his underlying utilitarianism but also the distinction, which he never forswears, between "rights properly so-called" and "political" rights, makes it problematic to read him as what we would call a 'libertarian'.
Older adults with poor vision or physical disabilities or serious illness may find it less problematic to read and complete the shorter version of the questionnaire (27).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com