In addition, 16S rRNA and single copy gene approaches may be problematic for differentiation among highly related strains (as these methodologies do not consider the influence of HGT).
Furthermore, it was hoped that comparative assessments would allow differentiation between problematic and less problematic active substances or formulations and rank them according to their risk potential.
Rheumatoid arthritis (RA), systemic lupus erythematosus (SLE) and fibromyalgia (FM) are usually easily discriminated on clinical examination, but have several overlapping features that make their differentiation more problematic in epidemiological surveys.
This issue is especially problematic for the study of perinatal differentiation.
In effect, in areas with low-medium read-depth, differentiation between SNPs is problematic.
But if it does not recognize the need for such a differentiation, it may indeed be problematic.
Furthermore, immunohistochemical studies (e.g., dot-like staining for epithelial membrane antigen typically present in ependymal tumors) and occasionally electron microscopy (for ultrastructural demonstration of ependymal or neuronal differentiation) may be helpful in problematic cases.
Identification of drug involvement and differentiation from other illnesses is problematic, although radiological manifestations and clinical tests enable many of the alternative causes of symptoms in advanced NSCLC to be excluded.
However, inferring the absence of population differentiation from this FST value is problematic.
However, when there has been prior pelvic surgery and/or radiotherapy, differentiation of fibrosis from tumour can be problematic.
