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There is comprehensive information in the literature regarding the humoral immune response of the female genital tract [9], [10] although the role of cervical antibodies in disease prevention for a number of STIs remains controversial in part due to their low levels and therefore problematic detection [11], [12], [13].
Eleven proteins were excluded from the original set due to problematic detection and two additional ones were outliers.
Additionally tests on randomly chosen predicted gene models often resulted in a problematic detection of the genuine N-terminus of the models especially of such proteins with unknown function and/or poor conservation.
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Directly detecting Bartonella in humans is problematic, but detection of antibodies as an indication of exposure to the agent has been widely used in previous studies.
Because the low abundance of small RNAs can be problematic for detection via northern blotting, some researchers have enriched small RNAs using LiCl or other methods (Song et al, [2012]).
Dried blood spots (DBS) are a useful specimen collection tool in situations where venous access is problematic, however, detection of biomarkers from this specimen type is subject to variation depending on storage conditions and storage time.
Biosensors are in perspective the best analytical tools for reporting the occurrence of chemical signals in the plant microbe interactions because generally, the effector molecules are released in trace amounts and localized in hot spots, and this makes problematic their detection with chemical methods.
This observation is problematic when detection of a specific RNA strand is needed.
This requirement may prove to be problematic for detection of slow-growing, or fastidious organisms.
The use of the KI-starch method is problematic for the detection of lipoxygenase producing transformants of metagenomic libraries, since the color reaction takes place under very acidic conditions.
Competition between viral strands and labeled probe makes nuclease protection assays problematic for the detection of strand RNA, particularly late in the infection when strands are much more abundant [3].
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