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However, benzene is not a problematic compound concerning the utilization of biomass gasification.
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The first step in creating a diverse library is to remove all the potentially problematic compounds.
Other potential problematic compounds are: ethylene, diborane and ethane (in this order).
These data show that there is a fairly low percentage of potentially problematic compounds in screening libraries.
A set of 5 SMARTS-based flags is also available to provide warnings on potentially problematic compounds that typically contain undesired substructure patterns.
These average values nevertheless mask some differences between providers, with some of them having up to 20%percentt of potentially problematic compounds.
In particular, failure to recognize and remove promiscuous and similarly problematic compounds can easily lead to expensive false trails, wasted resources, and flawed conclusions.
We note that the implemented real-time fluorescence monitoring (kinetic read) to derive reaction rates also makes the TAMRA/BHQ-2 assay less susceptible to variations in the screening equipment and permits facile discovery of problematic compounds which may interfere with detection [30].
Because of this high biomass concentration the rate of degradation is higher and specialists are grown for problematic compounds.
In addition to this manual approach, we applied computational filters such as Pan Assay INterference compoundS (PAINS) to identify potentially problematic compounds from structures (Supplemental Table 1).
These efforts could thus provide the opportunity to focus limited resources on the most important, problematic compounds (Dix et al. 2007).
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