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Mathematical models addressing the problem of receptor sorting into alternative endocytosis pathways do not currently exist.
Finally, we want to emphasize that the generality of our model makes it applicable to ultrasensitivity in signaling processes other than the here discussed problem of receptor sorting.
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We also used a constitutively active form of arrestin (R175E) to circumvent potential problems of receptor phosphorylation heterogeneity, which could complicate both the arrestin binding studies and receptor purification.
In relation to this, Stern warned of the potential problem of using receptor levels as a sole prognostic indicator, and suggested that phosphorylation-specific antibodies to detect ErbB2 activation could be more reliable.
The dissection of Wnt3a residues into sites essential for binding and for signaling will clearly suggest new approaches for tackling the problem of ligand-receptor specificity in the Wnt field.
GPCR-GFP fusions can also overcome problems of relatively low levels of receptor expression, the stoichiometry of the receptor and the fluorescent protein is well defined.
To conclude, the massive throughput superresolution data allows resolving reverse engineering problem to identify key biophysical features of receptor trafficking, which could not be previously addressed.
These include the design of replication-restricted viruses with altered receptor specificity, capable of circumventing the problem of loss of expression of adenovirus receptors on target cells, in particular CAR, by utilizing other, cancer specific proteins as alternate receptors.
The common mode of interaction of a large number of coactivators involving the LXXLL motif with most of the nuclear receptor raises the problem of a potential lack of specificity.
Up-regulation of SSTR expression by a priming administration could potentially reduce the problem with receptor saturation in SSTR-expressing tumors when large amounts of 177Lu-octreotate administeredered.
These hold both chemical and olfactory sensors in the form of millions of receptor cells.
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