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The only algorithm that was able to simultaneously solve both the non-linearity and the crowding problem in this dataset was ncMCE (Fig. 2A and E).
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We have previously investigated whether same source bias was an important problem in the dataset [ 19].
Departure of genotype frequencies of each MC1R variant from expectation under Hardy-Weinberg equilibrium will be assessed by Chi Square test among controls for each study, in order to detect any possible genotyping error or stratification problem in the datasets.
This variable was approximated by ability to handle unexpected and difficult problems in the dataset.
Completeness describes the seriousness of data loss problems in the dataset.
The absence of structural problems in the dataset and the assumption that animals behave independently (e.g. capture does not affect recapture probability) must be verified.
These observations clearly point to widespread problems in the dataset, which in the above examples are mostly likely the result of both mixtures/contamination and sequence alignment issues.
The bias error in S2 and S3 is well below the threshold of affecting the event grades, so this problem did not result in any loss of events due to the onboard grade rejection (as was the problem for chip I2 in this dataset).
Limited overlap in gene sampling among taxa could be highly problematic, irrespective of the amount of missing data per se, but this does not appear to be a problem in our dataset.
The ARCH effect represents the presence of autocorrelation and heteroskedasticity problems in the datasets.
These analyses suggest that bacterial and fungal contamination was not a serious problem in either dataset, but the true number of contaminating reads is unknown because 26% of the isogroups and 75% of the singletons remained unannotated (Table 5).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com