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BAC sequences present a simpler computational problem for assembly because there is less sequence to assemble compared to an entire genome from a diploid, outbred animal, and because a BAC is sequence from a single haplotype.
The problem for assembly that highly similar gene copies cause can to some degree be overcome by altering the k-mer size and the coverage cutoff as shown here.
Similar(58)
In contrast to similar problems for assembly lines, in transfer line balancing, tasks are grouped into blocks.
This indicates that gene repeats such as the surface antigens, which are driven towards diversity, were in many cases separated in the assembly; whereas highly similar repeats pose a larger problem for the assembly program.
Incorporation of Sanger sequences available in the public domain did not alter our assembly suggesting that the shorter reads generated by 454 sequencing did not represent a problem for correct assembly (data not shown).
This work focuses on a deadlock control problem for flexible assembly systems (FAS).
While this is not a problem in the context of whole exome capture, it may present a problem for accurate assembly of the resulting short-read sequence data.
However, it is to be considered that genome duplications in grapevine should not pose a big problem for the assembly of a physical map.
Because repetitive DNA is a known problem for the assembly of short pyrosequencing reads, and repeats are abundant in conifer genomes, we removed a large number of reads from the full data set to avoid sequences that could interfere with assembly.
The repetitive structure of the rRNA operons causes substantial problems for genome assembly software [ 42].
However, the combination of shorter read length and BAC clone pooling used in this study could cause problems for sequence assembly.
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CEO of Professional Science Editing for Scientists @ prosciediting.com