In a first step, we hypothesised and probed variables (confounders) that may be associated both with being born with an OFC (subgroups analysed separately) and with prescription of psychotropic drugs.
In order for the repertoire to be accurately measured, it is paramount to control any array fabrication and/or oligonucleotide probe variables that might lead to erroneously high or low signal intensities at any given address on the array.
As a measurement of importance of the 909 selected probes, variable importance of projection (VIP) values were calculated using the R library plsVarSel.
This is largely due to the lack of tools that can probe mechanical variables of the molecular length scales required for biochemical and cell signaling-based studies.
Empirical determination of Tm for optimal probe detection includes variables such as probe concentration, ionic strength and formamide concentration in the hybridization solution, time and temperature of hybridization and temperature and stringency of saline sodium citrate washes.
With most liquids probes, routine Variable Temperature operation over the range of +/-120 °C is available.
The functional groups, present on the modified electrode surfaces, were identified using cyclic voltammetry and ferricyanide ions, an electrochemical probe, at variable pH values.
A microarray consisting of oligonucleotide probes targeting variable regions of the 16S rRNA gene was designed and tested for the investigation of microbial communities in compost.
This study involved comparison of six ASO probes of variable length (25 to 9-mer), with a similar GC composition and designed to hybridise the target in a way that the mutation centrally occurred with respect to the duplex region.
(a) Northern blots for miRNAs using biotin-labeled probes and variable amounts of total RNA from Arabidopsis leaves.
The probes had variable spacing across the rRNAs, allowing characterisation of individual probe efficacy and probe spacing requirements.
