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We describe the design of a new protocol named T-TBP (temporally correlated ticket-based probing), which is extended from a previous proposal TBP (ticket-based probing) protocol [IEEE J. Sel. Areas Commun. 17 (1999)] that uses a spatial-oriented directed search framework.
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Methodological problems with the multiple probe protocol as utilized in most recent publications are discussed.
There was no effect of time passage across the repeated blocks of the one-probe protocol.
A modified dot-probe protocol has been shown to reduce SAD symptoms, in some but not all studies, presumably by modifying an attentional bias.
Participants were randomly assigned to a standard (i.e., control) or modified (i.e., active) dot-probe protocol condition and to participate in-lab or at home.
Major results: There was a difference in task demand as measured by reaction time between the two protocols (the multiple probe protocol was more demanding), and a difference trend in P300 detection sensitivity between protocols for both information types combined in favor of the 1PB (p < .07).07
The probes were labeled by a chemical reaction described in the Molecular Probes protocol (Invitrogen).
Positive clones were isolated and fosmid DNA was extracted and printed in 12×8 arrays on nylon membranes for hybridization with single overgo probes, protocol as above.
Anti-Rb G3-2455, BD) antibody was conjugated to the Alexa Fluor by mAb labeling kits according to the supplier's (Molecular Probes) protocol.
This so-called dynamic-probe protocol consisted in a two-step cycle aiming to maintain BFP transducer around zero-force, exerting no force on the bead, then no force on the cell protrusion according to principle of action and reaction (Fig. 2A B, Movie S3).
However, the co-engagement induced a clear decrease of the protrusion length emitted in the pushing phase; averaged maximal length obtained according to the dynamic-probe protocol dropped to Lmax = 3.1±0.6 µm, i.e. less than half value obtained upon engagement of TCR-CD3 aLmax (Lmax = 8.2±0.7 µm).
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