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Immunoprecipitated DNA was subjected to qPCR analysis using hydrolysis probes (see Supplementary data for Primer/Probe sequences).
Primers were designed from the barley sequences used for microarray probe design (Supplementary Data 2, available online).
Experimental details and probes are described in Supplementary data and Supplementary Table 3. PFGE was performed as described (Kobayashi, 2003); see Supplementary Materials and Methods for a detailed protocol.
Probe sequences are available in Supplementary Data 1.
Primer and probe sequences are detailed in Supplementary data, Table SI.
All the primers and probes designed for qPCR are provided in supplementary data (File S1: Table S3).
Sequences for the shRNAs are in Supplementary Data 6.
Primers used for FAIRE are listed in Supplementary Data 3.
Data for 20b– d given in Supplementary data.
We submitted a list of 45 Affymetrix probe-sets for 30 CREB-regulated genes (see Table S1 in Supplementary Data) using default query options with a shorter promoter length (-1000 bp upstream, 0 bp downstream).
The conservative and comprehensive lists of significant probes, regions, genes and GO terms are available as supplementary data (Data Set S1).
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