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Finally, the magnetic field is perfectly calibrated, with constant amplitude for every tested frequency, and with its known phase chosen as reference for the probes subsequent oscillations.
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One experienced dentist rated all restorations under magnification loupes with mirror and probe subsequent to a brief soft tissue survey and recording of gingival conditions.
Once we identified the first set of vertebrate sequences using the human probe, subsequent searches were conducted by using the sequences of representative species within a given taxonomic class or order to identify additional sequences in the same group of taxa.
Hybridization with DIG-labeled cprA DNA probes and subsequent detection with NBT/BCIP were carried out according to the manufacturer's instructions.
All procedures for preparation of labelled cRNA probes and subsequent Genechip hybridisations were performed according to suggested Affymetrix guidelines (http://www.affymetrix.com).
In spite of this, by default we opt to remove these probes from subsequent analysis.
To ameliorate this chemists will need to use computational ligand docking approaches and/or synthetic production of large numbers of probes for subsequent screening of highly selective probes.
Preparation of the labeled cDNA probes and subsequent Genechip hybridizations were performed in accordance with the Agilent Technologies One-Color Microarray-Based Gene Expression Analysis guidelines, as previously described [ 10, 11].
The combination of detection by internal hybridisation probes and subsequent melting curve analysis expands the spectrum to include mutation analysis by rapid genotyping.
All of the procedures for the preparation of the labeled cRNA probes and subsequent Genechip hybridizations were performed according to the Agilent Technologies One-Color Microarray-Based Gene Expression Analysis guidelines.
The whole reaction can readily be multiplexed through the application of a cocktail of probes and subsequent visualization of the individual PRINS products with color coded identifier oligonucleotides.
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