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We only tested multiple probes in the current study.
Only 22 of the 910 probes (19 would be expected by random chance) that attained genome wide significance with respect to smoking in Dogan et al. were ranked among the top 10,000 most highly associated probes in the current study.
The DAF-FM fluorescent probe permits analyses of NO in various cell types including skeletal muscle [29,32,33] and its use together with other ROS-sensitive probes in the current study has demonstrated an increase in NO in contracted skeletal muscle fibers from old mice in comparison with those from adult animals.
In contrast to that of smoking where the differences of methylation at cg05575921 between chronic smokers (~60%) and non-smokers (92%) can exceed 30%, the average differences at the more highly significant probes in the current study tend to be in the 5 to 10% range.
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"Although the pressures and densities probed in the current experiments are immense, nature is even more ambitious.
We also note that the non-adhesion between the substrate and the probe in the current simulated system also contributes to the ultra-small scratching force.
It is also correct that the number of potential spindle organizations that could possibly exist is far larger than has been probed in the current study.
This direct dissociation process occurs at lower temperatures than we have been able to probe in the current TPD experiments, but it is an intriguing implication of the calculations that we hope to examine in future studies.
Similarly, probe items in the current trial could not be from the preceding two trials.
Of the 278 significant probe sets in the current study, 249 (90%) were common to all three experiments.
It is possible that the use of multiple manipulation check tasks (i.e., both word and face visual probe tasks) in the current study lead to participant fatigue and reduced the sensitivity of individual measures.
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