Summarized gene antisense transcript abundances were derived similarly, using the probes from the antisense strand60.
The evolution of chromosome structures in Brassicaceae has previously been traced through comparative 'chromosome painting' techniques using BAC-size sequence probes from the A. thaliana genome26.
Transcript abundance was detected using quantitative PCR with TaqMan Gene Expression Master Mix (Life Technologies, California) and probes from the Universal Probe Library (Roche, Switzerland).
In addition, the probes from the DRX-500 (e.g., the TBI with a fixed 13C channel) can also be used with this instrument.
These results were consistent with the lower donor/acceptor ratio of the labeled probes from the FRET result.
This article describes NIR fluorescent probes from the point of view of chemical design, reaction mechanism, and targeting species.
After template extraction, the film was highly porous and enabled fast mass transport of redox probes from the surrounding solution to the electrode surface.
To allow a more thorough study of t(15 17), we designed small (0.9 3.6 kb), target-specific, single-copy probes from the human genome sequence.
In this study, to identify the HCV genotypes, an oligonucleotide DNA chip was designed using 15 probes from the 5′-untranslated region.
In the RIDA-LF combined assay, chemically labeled probes are covalently conjugated to magnetic microbeads, which is propitious to separate cleaved probes from the reaction solution.
The liberated target Hg2+ initiated the next cycling, thereby causing the conformational change of numerous hairpin probes from the stem-loop DNA structure to single-stranded DNA.
