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One great advantage of custom made arrays is that they enable total control over chip content with regard to probes for expression measurements, control probes and the distribution of probes over the array.
To identify those miRNAs that are differentially expressed during replicative senescence of BJ fibroblasts, we utilized a miRNA microarray platform that probes for expression of 470 human miRNAs and 64 human viral miRNAs, based on the Sanger miRNA database version 9.1.
Similar results were obtained [ 33] using the same probes for expression profiling of Ascochyta blight response in chickpea.
We therefore incorporated probes for hybridization-based SNP genotyping onto the microarray, taking advantage of available space left over after the design of probes for expression profiling.
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We used the daughterless-Gal4 driver to ubiquitously express PAX-FOXO1 and then probed for expression of a YFP-tagged embryonic D-Mef2 reporter (Cripps et al. 1998, 1999).
At the same time we also probed for expression levels of another GPCR, CXCR2, previously reported to be a regulator of DDR dependent cellular senescence14 along with its ligand, IL8.
Blots were probed for expression of the pan photoreceptor marker CRX and the rod photoreceptor markers recoverin and rhodopsin (Fig. 2D).
Our results prompted us to explore the possibility of utilizing BV displaying membrane proteins as the probe for expression cloning of receptor (or ligand) cDNA.
Human multiple tissue Northern (MTN) blots (BD Biosciences, Stockholm, Sweden), containing 2 µg poly A+ RNA each from eight cancer cell lines, were probed for expression of Tensin2 mRNA.
Breast cancer cell lines were probed for expression of hHR23a and 14-3-3σ 14-3-3σ 14-3-3σ2.
When TMA slides were probed for expression of BiP, CHOP and GADD34, each showed a significant relationship with histological subtype.
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