Exact(3)
PCA plot of samples was performed using all 1088 probes, by using a median centering of the data set.
A constant NO flux (162 pmol cm 2 s–1, 4.6 μmol cm 2 NO daily) was achieved from microdialysis probes by using a saturated NO solution as the perfusate.
The serum samples were later found to be positive for DENV-2 by multiplex RT-PCR with serotype-specific primers, and detection with serotype-specific probes by using a Luminex xMAP-based assay (Luminex, Austin, TX, USA) (unpub. technique).
Similar(57)
Non-linear effects, such as face wrinkling, have been probed by using a finite element method and the fidelity of the results assessed through comparison with measurements.
The STAT3 DNA complex was then separated from free probe by using a filter plate.
Real-time PCR assay was performed with target DNA, VCAM-1 primers, and a fluorescent probe by using a real-time PCR instrument (Applied Biosystems).
Unwinding was probed by using a 12-bp dsRNA substrate labeled with a fluorophore (6-carboxyfluorescein; FAM) and quencher (Iowa Black FQ; IBFQ) probes at the 5′ and 3′ ends, respectively (IDT).
Unwinding was probed by using a 12-bp dsRNA substrate labeled with a fluorophore (6-carboxyfluorescein; FAM) and quencher (Iowa Black FQ; IBFQ) probes at the 5′ and 3′ ends, respectively.
Alternate 20-µm thick coronal sections were collected from each brain from the rostral to the caudal end of the SCN or DMH (24 32 total, 12 16 for each probe) by using a Leica CM3050S cryostat.
HSA was probed by using a 1 30 000 dilution in PBS with 0.1% Tween and 2% bovine serum albumin (BSA) of the HRP conjugated Human Albumin detection Antibody (A80-129P, Bethyl) and detection was performed using the SuperSignal™ West Pico Chemiluminescent Substrate (34079, Thermo Scientific).
For a test patient sample, a series of ratios was generated for each diagnostic probe against 13 internal reference probes, rather than generating a single ratio for each probe by using an average of reference probes, to minimize differential tail-off effect.
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