Total RNA (100 ug), spiked with a bacterial-RNA mixture for control, was used to prepare direct Cy3- and Cy5-labelled first-strand cDNA probes using a single-base anchored oligo dT17 primer (Sigma, St . Louis MO, USA) and Superscript II reverse transcriptase (Invitrogen, Carlsbad, CA, USA).
Infinium II type probes use a single probe with a single fluorescent tagged base ligation that is specific for the methylated or unmethylated states of the CpG of interest.
Neural circuitry within M1 may be probed using single-pulse TMS using the amplitude of the resultant motor evoked potential (MEP) which reflects both cortical and spinal excitability.
The Northern blots were probed using single-stranded internally labeled DNA probes as described previously [ 6].
The Fe S covalency has also been probed using single-molecule AFM by measuring the mechanical stabilities of Fe(III)–thiolate bonds.
The PCR reactions were carried out the same as for the FISH probe amplification, using a single primer or combinations of two primers (14F/34R, 34F/14R, 34R/Tel, 14R/Tel).
Both "HX" (1H, X, and 2H channels) and "triple-resonance" (1H, 15N, 13C, and 2H) probes were implemented using a single transmit/receive coil and multiple-frequency impedance matching circuits.
In the most extreme case, when the probe model is built using a single study containing 4 6 samples and the training data involves the same transcription factor as the new data to be analyzed, signals in the new data may be subtracted away by the TileProbe background correction.
Probing for U6 was performed using a mix of DNA oligonucleotides complementary to U6, whereas probing for let-7 was performed using a single DNA oligonucleotide complementary to the mature let-7 sequence.
In addition, we examined the differences between profiling gene expression using a single probe at the 3' end vs. using multiple probes spaced along the gene.
