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An otherwise gracious waiter described what went into the wild-mushroom trifolati by saying, "You know, seasonal mushrooms"; when probed for specifics about the cheese plate: "Oh, there are all different types of cheeses, from all different countries".
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So a Western blot analysis was performed and probed for specific signal transduction molecules.
Membranes were then probed for specific signaling proteins using antibodies for detection of p70S6K1 and phospho-p70S6K1 (Thr 389).
FISH was performed on human skin 20 µm-thick microsections in situ or on freshly isolated nuclei, to probe for specific chromosome centromeres or loci.
However, not having the results when undertaking the interviews limited the ability to probe for specific results.
These SNP-based methods use genotype data to probe for specific genomic inheritance events that suggest inherited or de novo deletion polymorphisms.
Hence we probed for LC3, a specific marker for autophagosomes, to test if autophagic activity was induced by 17-AAG.
The interviewer probed for specific information relevant to the item, using both retrospective and concurrent probing technique (Willis et al. 1991, Tourangeau et al. 2002).
He probed for more details and specific examples when necessary.
Interviewers were also instructed to probe for a specific age if respondents first mentioned a broader range of ages.
Another constraint limiting direct imaging strategy is the necessity to develop a specific probe for each molecular target and then to validate the sensitivity, specificity, and safety of each probe for specific applications before their introduction into the clinic.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com