Sentence examples for probed 1 from inspiring English sources

Membranes were washed in TBST (0.1% Tween 20/TBS) and probed (1 hour) with HRP-conjugated secondary antibodies in Blocking Buffer.

Membranes were probed 1 hour at room temperature or overnight at 4°C with primary antibody, washed in T-TBS and incubated for 1 hour with fluorescent secondary antibodies.

The guide probed: (1) health status; (2) logistics of care abroad; (3) experiences abroad; (4) decision-making; and (5) ethical considerations.

Map 2 was probed (1 1000) mouse monoclonal antibody (Pierce).

Each condition was probed 1 4 times and averaged over 46 ROIs.

Blots were also probed (1 5000, overnight) with an antibody that recognizes β-tubulin-III for loading control.

Membranes that were probed >1× were stripped with Restore Western Blot Stripping Buffer (no. 21059; Thermo Scientific) and reblocked with 5% nonfat milk in PBS-T between immunoassays.

The separated proteins were transferred onto a PVDF membrane by semi-dry blotting and probed (1 2000 dilution, incubated overnight at 4 °C) with a rabbit polyclonal antibody directed against amino-acid residues 351 650 at the C-terminus of S. cerevisiae Yap1p (Santa Cruz Biotechnology, Santa Cruz, CA).

Lysosomal fractions were probed with (1 1000) rabbit polyclonal LAMP2a antibodies (Abcam), BACE-1 was probed (1 1000) with rabbit monoclonal antibody (Thermo Scientific), ADAM-10 was probed with (1 1000) rabbit polyclonal antibodies (Abcam), and presenilin-1 was probed with (1 1000) rabbit polyclonal (Cell Signaling).

Three DNA probes, a helper DNA probe (HP), hairpin probe 1 (H1) and hairpin probe 2 (H2) are ingeniously designed.

Probe #3 shows a higher sensitivity compared to probe #1 and it is superior to probe #2 because it operates in a fog-free condition.