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Hybridization of a genomic Southern blot to a radiolabeled probe verified that the entire donor plasmid integrated into the mosquito genome.
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After participants completed training, probes verified whether the conditional relationships learned were controlled by selection (e.g., if A1, select B1), by rejection (e.g., if A1, reject B2), or both.
Blot hybridization using the C-mos overgo probes verified that the plate and well address obtained from the filter were correct.
No cartilage lesions detected in MRI were considered an exclusion, but the final decision whether or not to include the patient in the trial was made at arthroscopy, where the meniscus injury (and commonly associated chondral lesions) were visually and physically (probing) verified and assessed.
The accuracy of the probe was verified by using an external temperature probe to manually check the temperature over a range of 20 60 °C.
The effectiveness of 9 in activation and formation of DNA ISC per probe was verified by comparing with dimers 5 8 of double activations with two probes.
Using this FRET-based probe we verified that 24 hrs after tet removal, RhoA activity, which was assessed as the ratio of the CFP signal to the YFP signal, was significantly reduced (Figure 6B).
Specificity of each probe was verified in a series of pilot ISH (method described in detail below) on paraffin sections of the transfected and untransfected HEK293 cells (data not shown).
The quality of the probe was verified by electrophoresis in a 1% agarose gel.
Authenticity of the probe was verified by restriction endonuclease digestion and partial sequence analysis.
Before implantation the calibration of each probe was verified according to the instructions given by the DSI manual.
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