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Application of multiple bioinformatic and experimental probe validation analyses consistently yielded 85 90% specificity for detection of designated targets, with 10 15% recognition of homologs from organisms within the same microbial family.
Consistent with our probe validation results, we analyzed the phylogenetic composition of the differentially expressed gene sets corresponding to clusters A through E (Fig. 5) at the family level (Fig. 9).
Panviral microarrays are useful for broad surveillance of bloodborne pathogens (9 ), yet rigorous individual probe validation across multiple targets is needed because of potential cross-hybridization artifacts.
To finally verify that our probe validation procedure was beneficial, we computed the Pearson's product moment correlation values for eggplant samples.
Single-stranded DNA oligomers were used as target DNA for probe validation, and each target DNA contained mismatch es), deletion(s), or insertion(s).
Sometimes (especially during target primer and probe validation runs), additional input quantities are included such as the minimum and maximum RNA amounts above and below the limit of detection to help differentiate between specific and non-specific products.
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These results were consistent with the bioinformatic and experimental probe validations described above, and indicated that the selected probe subset hybridized with specific functional targets >96% of the time.
This paper gives an overview of the concept design for IVVS as well as of the various engineering analyses and R&D activities carried out in support to this design: neutronic, seismic and electromagnetic analyses, probe actuation validation under environmental conditions.
This paper gives an overview of the various design and R&D activities in progress: plug design integration, probe concept validation under environmental conditions, development of a metrology strategy, the whole supported by a nuclear analysis.
It's a desperate tactic that allows you to probe for validation under the cloak of self-deprecation.
Sequencing of 25 putative polymorphism-containing amplicons yielded a SFP probe set validation rate of 68%.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com