Parameters, such as salt concentration and the lower-bound Tm of probes, are available for users to optimize their probe design query.
Another new function, "Unique Probe in the user-defined genome/ transcriptome," selects probes for target sequences using the genome/transcriptome sequence set (in FASTA format) as the background reference uploaded with the probe designing query.
Besides, one tendency in the post-genomic era is the fast growth of sequences deposited in public sequence databases or resolved in an individual lab, thus the increased target sequence entries will be submitted in a probe design query.
Although ERα gene amplification in breast cancers is controversial [ 41], we performed FISH analysis on tissue microarrays (TMAs) with known AR-positive breast cancers using a gene probe for AR and a centromeric chromosome X probe to query for AR gene amplification.
This array contains probes to query 51,279 transcripts representing two rice cultivars, with approximately 48,564 japonica transcripts and 1,260 transcripts representing the indica cultivar.
It's the poke, the probe, the veiled query, the grab for advantage.
A probe (query) data is again defined either as (i) a 3D object model (denoted or ) processed in order to extract multiple 2D silhouettes, (ii) multiple sketched contours of the same mental query (target), or (iii) simply 2D silhouettes extracted from multiple photos of the same category (see Figure 2).
The Gnutella protocol supports five types of messages: ping and pong used to probe the network, query and queryhit used to exchange data, and push used to deal with peers behind the firewall.
A batch-MegaBLAST was performed within the human refseq mRNA database from NCBI build 36.2 using the probe sequences as query [ 25].
The discrepancies were traced back to (i) annotation problems: Hmga1, Lin28, Phip, (ii) different length of 3'UTR sequences where location of the microarray probe would not query all transcripts arising from the respective genes: Sema3a, Rod1, Slc2a1, or (iii) alternative splicing: Ogt, Tfrc [ 26], Creb1 [ 27].
The rice genome array (www.affymetrix.com), designed for gene expression analyses, and contains probes to query 49,824 transcripts representing two rice cultivars with 48,564 from japonica and 1,260 from indica cultivar.
