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To probe the function of postnatal progenitors, a conditional mutant allele of Foxn1 was reverted to wild-type function in single epithelial cells in vivo.
Once this is achieved, the entire repertoire of yeast genetics is available to probe the function of the gene, or to engineer the expression in useful ways.
When combined, these techniques make it possible to probe the function of the lateral-line sensory system in an intact zebrafish using controlled, repeatable, physiological stimuli.
These studies demonstrate the power of the CRISPR/Cas9 system to probe the function of putative oncogenes and tumour suppressor genes in mouse models more rapidly than previous approaches.
Single molecule techniques such as optical trapping(8), atomic force microscopy(9), and magnetic tweezers(10,11) allow researchers to probe the function of enzymes at a molecular level by exerting forces on individual proteins.
The BMM summer course includes tutorials on general theoretical foundations and computational methods used in intelligence research, with hands-on computer work, and introductions to empirical methods used in neuroscience and cognitive science to probe the function of neural circuits and emergent behavior.
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Future studies should probe the functions of non-CpG methylation during the development and progression of breast cancer.
The present work sets the stage for further experiments to probe the functions of the proteins.
Here, we use micrococcal nuclease (MNase) footprinting to probe the functions of two chromatin-remodeling complexes.
Even CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats), the basis of the genome editing technology that drastically changed the prospect of gene therapy for the better, would not exist if microbiologists were not curious enough to probe the functions of the bizarre arrays of repeated DNA sequences they found in bacteria.
Therefore, we devised a series of experiments to probe the functions of the de novo proteins.
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