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The aliens are wide-eyed, ghostly puppets, who spin Arias on the wheel — or "probe table" — and pump him for information about life on earth, as he claims to be a virgin and pleads with them not to "pop my cherry".
To examine this structural dissimilarity, we built a similarity matrix based on compounds' MACCS keys and using abacavir as the active reference probe (Table 1).
Hybridization was performed as described in Adachi et al. (2009) using an 880-bp fragment amplified with hph-specific primers (HS-1com1 and HAS-2com) as a DNA probe (Table 1).
Microarray and qPCR data were in good agreement in terms of separating upregulation and downregulation, even if this opposite pattern was observed in different tissues but of the same probe (Table 3).
Fig. 2 Block diagram of the sensor probe Table 1 Model numbers of devices contained in the sensor probe Device Model number Power consumption CPU dsPIC33EP512GP504 <400 mW Wireless communication WH-MZ2 (Oki Electric Industry Co., Ltd).
Five primer pairs designed to amplify a 45 60 bp segment within each probe (Table 4) generated fold change estimates that were in general agreement with the direction (up- or down-regulation) of the raw signal data from the microarrays (Figure 3).
These kits were designed to target one specific gene of each causative pathogen with one specific primer pair and one shared-stem molecular beacon probe (Table S4).
All but one of the ST10 complex strains in our EAEC collection, and all of the Nigerian isolates, hybridized with at least one pAA-derived probe (Table S4).
Quantitative, real-time RT-PCR (qRT-PCR) was then performed [33] using alsSD-specific primers and a corresponding TaqMan probe (Table 2).
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The method uses an internal standard probe (IS-Probe, Table 1 and Figure 1) in the cytochrome b region of the genome (Koch et al, 2001), to estimate total copy number for mtDNA (i.e. deleted and wild type).
The level of 3895 bp deletion is determined by a probe 3895-probee, Table 1 and Figure 1) which spans the break point of the deletion ensuring that it is only amplified if the deletion is present.
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