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The amount of fluorogenic peptide probe sourced from either of two (thrombin or HIV-1 protease) commercial biochemical activity assay kits intended for ∼100 microplate-scale reactions is sufficient for 15 droplet-scale screening campaigns, performing ∼50 million total assay reactions.
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Each microarray includes approximately 15 k features containing probes sourced from the miRBASE public database [ 19].
The new specialized and standardized transcriptome-wide sheep microarray (Agilent Technologies) contained 15,008 sheep probes sourced from RefSeq and UniGene.
Each microarray includes ∼15-k features containing probes sourced from the miRBASE public database, release 13.0 (Griffiths-Jones et al, 2008).
The 43,803 porcine probes sourced from UniGene (Release 33, Feb 2008), RefSeq (Release 27, Jan 2008), TIGR (Release 12, Jun 2006) using Agilent 60-mer SurePrint technology.
For tissue samples assessed at the UK site, these probes were sourced from Empire Genomics LLC, Buffalo, NY; for tissue samples assessed at the China site, the probes were generated in-house.
This microarray consisted of 60-mer DNA probes for 470 human miRNAs, sourced from the Sanger miRBase public database (Release 9.1).
The appropriate secondary antibodies were sourced from Jackson Immunoresearch and Molecular Probes.
The miRNA array contained the complete content sourced from Sanger database 12.0, i.e. 851 probes for human and 88 probes for viral miRNA transcripts (Agilent, Santa Clara, CA, USA).
Sourced from: The Belfast Telegraph.
Sourced from the New Zealand Herald.
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