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The homogenized solution was immediately subjected to high intensity probe sonication (Vibracell VCX130; Sonics, USA) for 5 min.
An aqueous solution of FITC (V = 1 ml, C = 1 mg/ml) was emulsified in solution while vortexing for 20 s and further sonicated using probe sonication (ultrasonic liquid processor, Misonix, USA) at 10 W for 1 min to form primary w/o emulsion.
However, probe sonication (Fisher Scientific 120 W Sonic Dismembrator) yielded reproducible results.
The organic phase was emulsified with 4 ml of pH 7.4 phosphate-buffered saline containing PVA 205 (3 %, w/v) by probe sonication (VC 505, Vibra-Cell Sonics, Newtown, CT, USA) at 45 W for 30 s in an ice bath.
The solutions were dispersed via probe sonication using a VCX-750 ultrasonic processor (Sonics & Materials, Newtown, CT).
Dispersion was by probe sonication on ice with 3 4 bursts of 15 sec duration each (W220F Sonicator, 40 watts power).
Probe sonication.
There are two sonication techniques: a) Probe sonication.
The organic phase was mixed with the aqueous phase by probe sonication for 5 min.
The experimental results show that thermal conductivity increases smoothly with probe sonication time.
Amongst the two sonication processes, probe sonication process produced well stabilized small sized particles.
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