To substantiate this conclusion, we next combined IF microscopy with fiber-FISH, using an alphoid satellite DNA probe (pan-centromere probe) to directly visualize centromere DNA (Dunham et al. 1992; Baird and Farr 2006).
It's not clear how the Amazon probe will pan out but it signifies a stepping up of the Commission's action in this area.
Based on known CDR3 sequences of γδ T cells, we generated cell lines expressing specific γδ TCR, which may be used as a probe to pan antigen peptides recognized by γδ TCR.
Recently, a novel strategy that used intact cells as probes to pan target peptides in a phage-display library was developed [18] [19].
In our study, we first applied the transfected cells as probes to pan the phage library for screening of the epitopes recognized by γδ TCR.
In the present study, we established a new approach to screen epitopes or protein antigens recognized by the γδ TCR using Bacillus Calmette-Guérin (BCG-) specific γ <img src="http://journals.plos.org/plosone/article/asset?id=info?doi/10.1371/journal.pone.0018809.e001.PNG" class= inline-graphic"/> TCR transfected cells as probes to pan a 12-mer random-peptide phage-displayed library.
Equal loading was confirmed by probing with pan-actin monoclonal antibody (AC-15, ABCAM, Cambridge, MA, USA).
Probing with Pan-cadherin antisera revealed an increase in cadherin membrane translocation, consistent with a previous report that S1P strengthens adherens junction formation [ 40].
Blots of recombinant α-syn incubated with distilled water (α-syn) casein kinase I (α-syn + CKI) or casein kinase II (α-syn + CKII) and probed with pan-α-syn (column 1), pSer129 α-syn (column 2) or pSer87 α-syn (column 3) antibody.
The Real-GP®, -GN®, and -CAN® real-time PCR kit (M&D, Wonju, Republic of Korea) assays use the TaqMan probes for detecting pan-GP, pan-GN, and pan- Candida species, respectively.
Two probes were utilized on separate pieces of mucosa - a S. aureus specific 16S sequence conjugated to Alexa-488 probe, and a pan-fungal 18S Alexa-488 probe.
