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The minimal requirements of probe number are also presented to provide guidelines for BTT system design.
The array represents 106,285 probes, which is more than double the probe number of the currently available 44k microarray.
The task was to decide whether a single probe number had been shown as part of a previously presented pair of cue numbers.
We developed parameters, thresholds, and testing conditions that could be used to differentiate various systems that spanned detection chemistry and instrumentation; probe number and selection criteria; and sample processing protocols.
Behavioral data showed a clear interference effect (LeFevre interference), in that performance in the non-matching condition was significantly better when the probe number was arithmetically neutral compared to when it was arithmetically related to the cue digits either strongly (i.e., through the product) or weakly (i.e., through its neighbor in the multiplication table).
Probe intensities were averaged across all genes by probe number.
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Fig. 9 Relationship between simulation accuracy and probe numbers.
Gene-specific universal probe numbers and primer sequences are available upon request.
The massive increase in probes (∼5.6 million), along with exon length-dependent probe numbers, enable unprecedented resolution into these genomic units.
Sequences of newly designed primers and probe numbers of the Exiqon probe library system (Exiqon, Vedbaek, Denmark) are listed in Table 1.
Primers and UPL probe numbers are detailed in Table S2.
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