Exact(3)
After correcting for probe effect (by subtracting probe means), the gene means were determined from each probe set.
We chose to analyze the five probe means for each gene, rather than take an overall mean (or median), because our mixed-model approach enabled us to consider all this information in the model rather than discard the variation that may be present among different probes targeting the same gene.
By examining graphs of probe means vs. probe position in the probe set for a sample of probe sets, an Rsq ratio of four or greater was determined to give a clear indication of different patterns of hybridization that would be likely to be caused by a SNP.
Similar(5)
The digital thermometers come in both the "instant read" variety, involving a metal probe meant to be used for a quick temperature check but not left in the oven, and another type that allows the probe to be left in the bird while it cooks.
Results such as sample probe mean, standard deviation and z-scores are returned with the sex predicted for each sample.
For each probe, mean values were calculated for each bird-pair within the microarray to remove the dietary variable prior to between-tissue comparison.
Rectal temperature (TH-5; Physitemp Thermalert, Clifton, NJ, USA; with a rat size thermal probe), mean arterial blood pressure (MABP), and heart rate (HR; 2001A, Datascope Corp ,Paramus, NJ, USA) were continuously monitored.
Despite this, a corresponding reduction in total c-Jun immunoreactivity associated with the SOCS3 ΔAprobeobe meant that the ratio of pc-Jun (Ser 63):total c-Jun was relatively unchanged (Fig. 2; lower panel).
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