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Probe lines were infected with avirulent PstAvrB (107 cfu/mL) and mock infiltrations were used as a control.
INNO-LiPA HCV 1.0 contains two control lines and 19 5'NCR DNA probe lines specific for the different HCV genotypes and subtypes.
INNO-LiPA HCV 2.0 contains the same lines, plus an additional core control line and three core DNA probe lines, specific for HCV genotype 1 subtypes and genotype 6.
The HPV genotyping strips contained 29 probe lines plus one reference line, detecting 27 individual HPV genotypes and two concentrations of the β-globin control probe.
The hybrids were detected with alkaline phosphatase streptavidin conjugate and substrate (5-bromo-4-chloro-3-indolylphosphate and nitroblue tetrazolium), resulting in a purple precipitate at positive probe lines.
After hybridization of the PCR product to the probes on the strip under stringent conditions, followed by stringent washing, the hybrids were detected by alkaline phosphatase/streptavidin conjugate; reaction with a substrate (5-bromo-4-chloro-3-indolylphosphate and nitroblue tetrazolium) results in a purple precipitate at the positive probe lines.
Similar(53)
At 2230 hours, Burden was found on the first pass of the probe line 60 feet above her ski and pole.
Through a two-step assembly process, these spiral channels intersect with pre-immobilized radial DNA probe line to form an array of DNA that covers the whole CD surface.
The lowest probe line was positioned along the Fp1-Fp2 line and the center probe was located at Fpz).
Positive hybridization was detected by streptavidin-horseradish peroxidase-mediated colour precipitation on the membrane at the probe line.
Any sample positive for the HPV 52/33/35/58 probe line on LA are further tested to confirm the presence or absence of HPV52 [ 34].
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