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P.E.B. and M.J.W. provided the small molecule epigenetic probe library.
Specific primers were designed using the universal probe library assay design centre and checked with BLAST (Supplementary Table 2).
We have explored a novel molecular framework with a lysine core to prepare a probe library for protein kinases.
The epigenetic probe library was obtained from the Structural Genomics Consortium (University of Oxford, http://www.thesgc.org/chemical-probes/epigenetics, version as available in May 2014).
Probe library design would benefit from a general purpose tool for analysing these issues.
The primers and probes were designed with the universal probe library (Roche).
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For one method we constructed a promoter-probe library by fusing ΔSterne genomic fragments to a promoterless GFP gene.
Primers and probes were as follows: For nuclear DNA, primer-1, 5′-TCTTCTGGACTGTGAACCTGTG-3′; primer-2, 5′-CTGGTGGGAAAGATGACCAC-3′; and probe, Universal Probe Library (UPL 5959 (Roche).
PCR contained normalized cDNAs, Universal Probe Library Set, and FastStart Universal Probe Master (Roche, Basel, Switzerland).
Primer and probe sequences were designed by Universal Probe Library (Roche) as listed in Table 4.
Primer sequences and Universal Probe Library probe numbers were mouse Avpr1a, no.
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