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Node/antinode patterns along the probe length were observed, as was an elliptical motion along the length of the probe.
To this end, the electrochemical response was monitored versus the DNA target length, for a constant DNA probe length.
The best strategy allowed probe length to vary within 32 40 bp to equalize hybridization free energy.
This required a careful optimization of the ternary complex architecture as well as adjustments of probe length and probe reactivities.
Probe length has two important consequences.
Probe length ranged from 323 1200 nucleotides.
Discrimination is strongly dependant on probe length, and decreases dramatically with increases in probe length [3], [6] [8].
These libraries require huge numbers (approaching 4N) of probes for relatively short probe length.
Our central finding is that the optimal probe length varies significantly from target to target.
Note that eqn. (17) does not depend on the probe length l.
Tm, probe length, and GC content all appear to be related to one another.
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