Spectroscopic properties of the fluorescent probe were consistent with predictions for relative solvent accessibilities; however, even the less solvent accessible probes reported a quite polar environment, suggesting that this region of the apo-protein is either substantially solvent exposed or undergoes significant dynamic motion.
Indeed, gene expression associations with this probe are consistent with those from ILMN_1710903, which should recognize all transcripts, although ILMN_1710903 is confounded by a confirmed variant within its sequence.
Our observation of high expression "silent" probes is consistent with a probe cDNA hybridization model where all probe-binding sites are occupied.
As shown in Figure 2a, the peptide detected upon array probing was consistent with the epitope specificity designated in the product data sheets provided with the various antibodies.
Based on a comparison of genotyping calls between two replicates (only two of the M0023 Cy3 replicates was used to enhance the consistency of analyses) the majority of the genotype assignments determined using B>M probes were consistent between pairs of replicates and only 2 4% of probes were called as different genotypes in the two replicates.
In the accession with highest genome size as measured by flow cytometry, Loh-0, we found more than a two-fold increase in 5S RNA gene copies relative to Col-0; results from fluorescence in situ hybridization with 5S probes were consistent with the existence of size polymorphism between Loh-0 and Col-0 at the 5S loci.
The lack of variation in the effect of ligands on probe binding is consistent with high off rates for MSH4 in both monovalent and oligomeric constructs relative to that of the competing probe.
NMIA and kethoxal footprinting of probe 9 is consistent with strand invasion that breaks P8.
NMIA and kethoxal footprinting of probe 3 is consistent with strong binding to stem P7 inducing a rearrangement of stems P7 and P8, which results in formation of new base pairs, G22-C154, C23-G153, A25-U151, G27-C149, G27-C149, and perhaps G28-C148.
The clinical group, not only showed signs of clinical improvement but also increased running speed to the MID and NR+ probes, which is consistent with the "optimistic" affective bias predicted for an antidepressant.
