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We propose a sequential series of considerations when designing probes that could significantly reduce probe failure (Figure 3).
As microarray probe density continues to increase, a 2% probe failure rate represents a substantial cost in data loss.
Thus we propose that the data reported here will help reduce probe failure and allow for maximum data extraction from microarray experiments.
No specimen yielded rif-resistance associated with more than one probe failure (mutation combinations).
In particular, speakers reported higher rates of clinical sample failure (from 10%to70%0%) than are typical for fresh-frozen tissues, as well as more frequent probe failure for individual samples.
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We tested for positional bias and showed that a biotin conjugated sense primer mirrored the same probe failures.
The high-energy gamma probe failures were in patient 2 in whom the total amount of injected radioactivity was very low (≈ 1 MBq).
Allowing some amount of false positive probes and some amount of false negative probes makes the HMM more robust to probe failures.
In selecting gene sets to assay, higher values for redundancy may be chosen to better accommodate probe failures, but such failures will result in decreased accuracy.
However, recently a variant of C trachomatis with a 377-bp deletion in the cryptic plasmid (GenBank accession no. EF 121757) has emerged, causing probe failures for certain commercial kits which target this deleted segment for their real time PCR assays [ 30, 31].
The council is probing failures among officials over the firms' collapse.
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CEO of Professional Science Editing for Scientists @ prosciediting.com