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We define θ as the inclination angle of the probe deviation from the vertical direction.
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Mean ICP differences between the various probes, standard deviation and limits of agreement were reported in most papers.
The mean within-pair correlation (r) was 0.99 across all analyzed probes on the array and 0.96 across all 'variable' probes (i.e. excluding those with a probe-wise standard deviation smaller than the estimated standard deviation across all probes on the array).
A mean normalized peak height was calculated for each probe and standard deviation established.
Results such as sample probe mean, standard deviation and z-scores are returned with the sex predicted for each sample.
All remaining data points were used for the calculation of the mean average signal for a given probe, and standard deviation for each probe was calculated.
For each probe, the standard deviation between the pre-treatment samples and, separately, the post-treatment samples were assessed against probe position in the target gene, number of concurrently probed transcripts, and the number of exons in the target gene.
To explore visit variability (intra-individual), we calculated Spearman rank sum correlations between the two visits across all probes after applying two common probe filters (standard deviation (SD) and detection score).
Both UV and electron beam irradiation change rather feebly the wettability for all studied probing liquids with deviation of the contact angles in a very limited range, i.e. Δθ ~ 5 15°.
Probes with greater deviation than this between NC1 and yw were selected as candidate SFPs, consistent with expectations given the high level of DNA sequence polymorphism in Drosophila.
Unsupervised hierarchical clustering was generated using probes with standard deviation (s.d).≥1.≥1
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