We are interested in developing disruptive antibody technologies to probe and counter high consequence targets, and one aspect of this employs llama single domain antibodies (sdAb or nanobodies) to generate rugged ligands [30], [37], [38].
Martz probes; Belichick counters.
For detection of neutral fat stores, cells were grown on 96-well plates, fixed with 2.5% formaldehyde, washed with 1× PBS, stained with 10 μg/ml 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza- s-indacene (BODIPY 493/503; Molecular Probes), and counter-stained with Hoechst 33342 (Molecular Probes) for nuclei identification.
72 hr later, cells were fixed and BrdU positive cells were detected by phycoerythrin-conjugated anti-BrdU antibody (Molecular Probe, Invitrogen) and counter stained with 4',6-diamidino-2-phenylindole (DAPI).
Based on the measurements by the Galileo probe (1995 2003) heavy ion counter (HIC), the choice of trapped heavy ions is limited to carbon, oxygen and sulfur (COS).
A small size probe, consisting of a counter and a reference electrode encased in a glass body ending to a fine aperture tip and pressed onto the specimen, was used to apply cathodic galvanostatic pulses on positive plate battery samples.
After allowing for the materials to localize in the lymphatic system, the clinician uses a handheld scintillation counter (gamma probe) to locate the nodes-receiving primary drainage from the tumour via lymphatic vessels.
The most significant technical disadvantage with INFORM is the lack of dual fluorescence probes HER2 CEP17, which would counter the level of false positives achieved with this kit at its recommended threshold.
That is why several workers who gathered around the cask emerging from the pool at LaSalle wielded long poles with probes and Geiger counters; when they find an area of contamination, another worker with a mop on a long pole cleans it up.
