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We employed the orthogonal design approach to analyze 3 factors, namely, the blood vessel's volume, the average distance between probe center and the blood vessel system and the number of the selected part's branches at three different levels.
However, the point data measured with a typical coordinate measuring machine (CMM) have often been the data of the probe center rather than those of the object's surface, and this poses a great challenge in reverse engineering.
The primary change between electron probes is not only the probe center position, but we can also vary coherent wave aberrations in the probe such as defocus or spherical aberration, change the probe size by modifying the probe semi-angle radius, and also include relative tilt between the probe and sample by moving the probe center away from (vec {q}= 0,0)).
The key insight is that because highly converged STEM probes decay to zero quickly with distance from the probe center position, they can be cropped out of the full S-matrix in a highly accurate Fourier interpolation scheme.
The initial STEM probe generated from a 25 mrads semi-angle aperture at 80 kV is shown in Fig. 3c, with the probe center position shown in Fig. 3d.
To overcome this bias a heuristic algorithm was developed which uses the probe center base position to adjust raw intensity values.
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