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In the first step, we applied an SNR threshold on an individual probe basis, choosing the threshold such that false positives were minimized and true positives were maximized.
This direct linear relationship was confirmed by scaling experimental data to both values using Expression Console (Affymetrix, Santa Clara, CA) and observing the ratios on a probe by probe basis.
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After quantile normalization (using limma package in R) on the age arrays (25 young and 26 older subjects, Table S1), we first determined the number of genes significantly differentially expressed with age, by performing on a probe-by-probe basis, 24354 two-sample t-tests.
Briefly, the learning algorithm allocates the signal intensity of each genotype into two distinct clusters on a per-probe basis [ 24].
Differentially expressed features between treatments were estimated by least squares fitting of linear models on a probe-by-probe basis using the entire pre-processed dataset.
We applied the threshold across all three replicate hybridizations on a probe-by-probe basis to identify the genes expressed in each tissue with a high level of confidence.
We describe a proof-of-concept experiment wherein we used hybridization intensity changes relative to wild type on a probe-by-probe basis to detect a known deletion on chromosome 5 in an IR64 mutant [ 6].
A linear mixed-effects model [ 28] was used on a probe-by-probe basis to compare expression between groups accounting for the correlation between replicates or paired normal-tumor samples from a given subject.
Psychedelic drugs could even be used to probe the basis of consciousness, according to Solomon Snyder, one of Dr Griffith's colleagues.
We hope that President Obama will probe the basis for these assertions, as we have," the two wrote.
We sought to probe the basis of the regulation of developmental branch pruning by the EGFR.
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CEO of Professional Science Editing for Scientists @ prosciediting.com