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The high-resolution intensity distribution at the probe apex was mapped in a plane parallel to the probe base.
The chemical compositions of the oxide thin films were analyzed using X-ray photoelectron spectroscopy (XPS; Theta probe base system, Thermo Fisher Scientific Co., Seoul, South Korea).
However, the measured intensity of left spot was relatively strong than the right one, compared to the simulation results, which might be attributed to the processing defects of the probe structure and the non-parallelism between the probe base and the detecting plane.
Emissions from probe:probe complexes must be determined individually, as each probe base sequence has a characteristic ability to not only form hairpin structures and self-dimer hybridized complexes, but also to form parallel homologous structures stabilized and signalled by intercalated YOYO-1 molecules (Daksis and Erikson, unpublished observations).
For this chip design we looked at the ratio of forward and reverse strand intensities as a function of probe base pair composition (Additional file 2: Figure S1).
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Herein we designed a peptide-based probe based on the proximity-induced protein conjugation reaction.
A new Hg2+-selective reaction-based probe based on thiophosphinated N,N-diethylrhodol was developed.
We designed an oligonucleotide probe based on the DNA sequence.
d The structure of a multimodal QD probe, based on silica-shelled single-QD micelles [40].
A low cost confocal probe based on time difference measurement has been developed.
Herein, we report a novel fluorescent probe based on coumarin to detect cysteine (Cys).
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