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The TB test, called a line probe assay, costs less than $8 and detects mutations in bacterial DNA linked to drug resistance.
Line probe assay.
The presence of MTBC was confirmed by multiplex polymerase chain reaction (PCR) [3], Gene Xpert, and line probe assay (LPA).
The generation of reactive oxygen species was measured by 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescent probe assay.
The LC probe assay and the reference RT-PCR used almost identical detection probes, which bind to enterovirus specific amplicons.
These mutations so far could only be differentiated by using reverse hybridization line probe assay [32].
Quantification of cDNA was carried out using a fluorescent probe assay.
Amplification primers and probe (Assay ID Hs00430216) of Taqman gene expression assay (Applied Biosystem) were used for hMena.
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In cases in which circumstances eliminate the possibility of one of the two species, BurkDiff can be used as a single-probe assay for specific identification.
As expected, all the positive samples detected by hybridisation-probe assay were also positive for clamped-probe assay.
K-Ras mutations in two of these tumours were detected by hybridisation-probe assay and three others by clamped-probe assay only.
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