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Similarly, FURA-2, a probe applied to lysates to measure calcium levels, fluoresced proportionately to IAM dosage.
In contrast, there was no difference between NR2B mice and normal mice in their response to acutely painful stimuli, such as a heating probe applied to the tail.
Direct probe pressure with high-frequency linear array ultrasound probe applied to our patient's quadriceps muscle produced swirling of the heterogeneous deep-muscle purulent material.
The fluorescent probe applied is CellROX® Green (CrX; Life Technologies, Eugene, Oregon, USA), which becomes fluorescent after oxidation and subsequently binds to DNA.
The probe applied to the slide under a glass coverslip.
For comparison, we can consider the total quantity of probe applied to the cells for imaging.
Similar(42)
For each cycle, the software determined the fluorescent signal from the VIC- or FAM- labeled probe (Applied Biosystems).
The detection system using the quantitative PCR probe functions in a manner similar to the TaqMan probe (Applied Biosystems).
Taqman master mix and CHK1 taqman probe (Applied Biosystems) was used for the real-time PCR reactions and 18s was used as an internal control.
Cells were washed with PBS for 15 minutes and then hybridized to a Cy-3-labeled (CCCTAA)3 PNA probe (Applied Biosystems).
Telomere fluorescence in situ hybridization (FISH) was done by using a Cy3-conjugated telomere-specific peptide nucleic acid (PNA) probe (Applied Biosystems, Framingham, MA, USA).
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