For unpredicted negative MOL-PCR results (SAL-20, SAL-38, SAL-47, SAL-58 and SAL-71), mismatches were observed in the alignment of the PCR amplicon sequence and the target-specific sequence of the upstream and downstream probes, which prevented adequate annealing of the probes and explained why no ligation occurred.
Interviewers often probed respondents to elaborate on and explain their responses.
The fundamentals on the probe operation are presented and explained in the presence of a metallic part to be inspected with and without defects.
Later, we were surprised and pleased when Louis Silverstein, the art director, told us that at a meeting of business executives called by Punch, the new publisher had asked probing questions, requested memos and explained that he would shortly issue his conclusions.
Frances P. Downes, a lab director at the Michigan Department of Health, helped investigate that outbreak and explained the procedures followed in such a probe.
For the remaining DEC probe sets that were located within the designed probe selection region, no other measure of probe set length or location was identified that was significantly different from INV probe sets and could explain the enhanced sensitivity to RNA degradation.
A formal probe would help explain how Metro-North, Consolidated-Edison, Amtrak and local, state, and federal officials can work together to prevent an infrastructure failure of this scale from happening again.
This observation suggests that differential expression between ontogenetic phases rather than poor probe quality might explain why a relatively large number of probes were excluded.
"A motor drives the probe down," he explained, and a sensor at the tip measures how much resistance it takes to get the probe through the varying layers of snow.
Preferential ligation of one allele during probe annealing could explain the occurrence of an artificial homozygous cluster.
